Testing for hepatitis B virus core antigen and e antigen may confer additional safety of donors′ blood negative for heptitis B virus surface antigen
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چکیده
منابع مشابه
Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli
DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...
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Background/Aim: Hepatitis B surface antigen(HBsAg) is routinely detected qualitatively in Hepatitis B Virus(HBV) infection where its persistence beyond 6 months defines chronic hepatitis B(CHB) infection. Hepatitis B e antigen (HBeAg) usually indicates active HBV replication and risk of transmission of infection. In spite of the emerging use of chemiluminescence based quantitative HBsAg assays ...
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BACKGROUND Nucleic acid test (NAT) hepatitis B virus (HBV) screening for all blood donations with a sensitivity limit of 25 IU/mL in the individual donation is mandatory in Switzerland since 2009. The aims of the two studies were to define the percentage of antibody to hepatitis B core antigen (anti-HBc) or anti-HBc and antibody to hepatitis B surface antigen (anti-HBs)-positive donors bearing ...
متن کاملAnti-HBc and HBV-DNA detection in blood donors negative for hepatitis B virus surface antigen
Occult HBV infection is defined as the presence of hepatitis B virus (HBV) DNA in blood or liver tissues in patients negative for Hepatitis B surface Antigen (HBsAg). Those patients may or may not be positive for HBV antibodies. The objective of this study is to determine the presence or absence of HBV DNA in the serum samples from HBsAg negative blood donors. In addition we aimed to assess the...
متن کاملoccult hepatitis b virus infection among blood donors with antibodies to hepatitis b core antigen
diagnosis of hepatitis b is routinely based on of serological assay of hepatitis b surface antigen (hbsag). occult hepatitis b virus (hbv) infection is generally defined as the detection of hbv -dna in the serum or tissues of subjects who have negative test for hbsag. transmission of hbv infection has been documented from hbsag negative, anti-hbc positive blood and organ donors. the aim of this...
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ژورنال
عنوان ژورنال: Asian Journal of Transfusion Science
سال: 2014
ISSN: 0973-6247
DOI: 10.4103/0973-6247.126700